Enzyme purification and related techniques

About this book

Buffers of any desired pH can be prepared quickly by mixing together the component stock solutions of a suitable buffer acid and that of its conjugate base in appropriate amounts according to Eq. (1), where the (1) pK represents the apparent pK at the ionic strength of the buffer and fd is the degree of dissociation, i.e., the fraction of total buffer in the dissociated form, A.