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Modern plant cell biology involves the integration of structural studies with biochemical and molecular analyses, allowing a detailed characterization of growth and differentiation, and the ways in which plant cells respond to a diversity of external and internal stimuli. This book discusses both established techniques used in the field and new advances which are already having a major impact. It begins with detailed descriptions of the latest optical techniques for viewing living plant cells. This is followed by chapters covering a wide range of modern methods for plant cell analysis including in situ hybridization, immunocytochemistry, enzymic cytochemistry, and the use of vital probes and stains. Confocal microscopy and electron microscopy are also covered, as are methods for microsampling and the measurement of components within single cells, electrophysiology, and the isolation and analysis of plant organelles. . Plant Cell Biology: A Practical Approach provides both newcomers and experienced researchers with a unique and comprehensive practical guide to this subject. It is a natural companion to Plant Molecular Biology: A Practical Approach.
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