Nonculturable microorganisms in the environment

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The term "nonculturable" was invoked by Xu et al. in 1982 (47) to describe starved but viable cells in a survival or dormant state of existence, observed to occur after an actively growing culture of Vibrio cholerae O1 or Escherichia coli was placed in a nutrient-free microcosm, e.g., a saline solution free of nutrient and incubated at low temperature.